NSI-189 Phosphate or Free Base Powder? Complete Nootropic Overview: Benefits, Side Effects, & More

 »  »  NSI-189 Phosphate or Free Base Powder? Complete Nootropic Overview: Benefits, Side Effects, & More


  1. What is NSI-189?
  2. How Does It Work?
  3. NSI-189 Phosphate vs Free Base
  4. NSI-189 & Depression
  5. Study & Development
  6. Benefits
  7. Side Effects
  8. Dosage
  9. FAQ (Frequently Asked Questions)
  10. Conclusion

Depression has spread widely and potently with countless people being affected and destroying their potential. This nootropic is regarded as a significant game-changer for treating depression and other brain illnesses. The information regarding NSI-189 is detailed here which cements it as an effective medication.

An antidepressant called NSI-189 functions differently from other antidepressants. According to researchers, this medication may be able to assist those who do not respond well to other treatments. Its benefits in boosting brain performance are still being studied.

What is NSI-189?

An experimental neurogenic substance called NSI-189 may be used to treat conditions like MDD, traumatic brain injury, stroke, issues with cognition, mental illnesses, post-traumatic stress disorder, and age-related neurodegenerative disease.

NSI-189 is being developed by the biopharmaceutical business Neuralstem Inc. The substance is being studied as a potential alternative medicine for individuals who did not respond to traditional drugs.

In addition to improving brain health, diabetic neuropathy, stroke, neurodegenerative disease, post-traumatic stress disorder, and Angelman syndrome, NSI189 is now still being evaluated for various disorders. The traumatic brain injury that it treats is used.

The hippocampus volume is increased by NSI-189, like other antidepressants. But what makes it unique is its capacity to identify the substances that are behind the aforementioned rise in rodent populations. Since a human clinical study is still required, the drug’s capacity to raise hippocampus volume makes it more effective against depression.

How Does It Work?

In neural stem cells generated from the hippocampus, NSI-189 has been found to encourage neurogenesis. The areas in charge of memory, learning, and emotion may also be impacted. In addition to other processes, this medication has the potential to cross the blood-brain barrier. A few more mechanisms that NSI-189 exhibits could support the claim that it is a more effective treatment for depression. NSI 189 expands the hippocampus region of the brain by 20% and modifies aldosterone receptors by enhancing glucocorticoid resistance.

NSI-189 Phosphate vs Free Base

When freebase and phosphate are both accessible, sometimes users are unsure which one to get. Both types exhibit enhanced cognitive performance while reducing depressive symptoms. To give additional clarity, here is what you need to know about their differences


Neuralstem Inc. uses phosphate in its clinical studies. It is most effective when taken orally, costs 15 to 20% more than the alternative formulation, but is significantly more stable and has superior bioavailability.


The sublingual ROA uses the freebase version. The nitrogen atom is attached to two carbon atoms, and one hydrogen atom, and forms the secondary amine. Its bioavailability is low.

To decide whether to get the free base or the phosphate while considering buying NSI 189, you may want to take into account the previously listed variables.

NSI-189 & Depression

After 28 days of treatment, those receiving NSI-189 significantly reduced their symptoms of depression compared to placebo in a phase I randomized placebo-controlled trial with 24 participants.

After 12 weeks of treatment, 220 participants in phase II randomized placebo-controlled trial compared the effects of NSI-189 (40 mg/day and 80 mg/day) with a placebo. Researchers’ expectations for this trial’s effectiveness in treating depression were not met.

However, patient-reported questionnaires revealed that depression symptoms significantly improved with the 40 mg/day dose in the phase II experiment. Phase I or II trials did not record any major side effects.

Phase I and II clinical trials that examined depression also identified some advantages for cognition. According to self-reported surveys, participants in the aforementioned phase I trial of 24 individuals taking NSI-189 showed improvements in cognition.

Similar to this, the 40 mg dose was connected to self-reported enhancements in cognition, including enhancements in memory, working memory, and executive function, in the phase II trial including 220 individuals.

Study and Development

In an initial phase 1B clinical trial, it was suggested that this novel agent not only improved the evaluation of mood but also assisted in the elevation of neurogenesis. However, these preliminary studies, which involved human subjects, were not conclusive as they were carried out in circumstances that limited the reliability of the results.

An investigation of the effectiveness of NSI 189 as a potential mood-improving treatment drug was done in phase II clinical study. It is hypothesized that this unique neurogenic chemical has no connections to the norepinephrine or serotonin reuptake inhibition pathways, which have a variety of effects, including mood.

A 40 mg oral dose that was administered orally produced favorable effects in terms of improved mood assessment. Additionally, it demonstrated a considerable improvement in CogScreen testing across a number of cognitive domains. In addition to improving cognitive symptoms and poor mood, this double-blind experiment also revealed no intolerance or major adverse effects, which is encouraging for a novel substance.

Another study identified NSI 189 as a neurogenic entity and found that it improved neurogenesis in individuals who were under a lot of stress, enhancing cognitive performance. However, these studies had significant drawbacks, including lower population size.

Recent years have seen a large number of studies using the mouse model that have produced positive outcomes for cognitive impairment. Although numerous mechanisms have been put up as the causes of these findings, many crucial components must still be present for it to be regarded as a successful therapeutic agent. The majority of currently available medications for mood enhancement do not appear to alleviate cognitive impairment, however recent research indicates that NSI 189 activation of hippocampus neurogenesis may.

The brain’s temporal lobe contains the hippocampus, which aids with memory and learning. It is thought to stimulate the hippocampus, which aids in both mood regulation and the stimulation of neurogenesis. In a different study, this tiny chemical demonstrated superior blood-brain barrier penetration in mouse models, hence more effectively treating neurodegenerative diseases. Additionally, it demonstrated a reduction in the neurological abnormalities brought on by a stroke in model mice.


What advantages does NSI 189 offer you? Researchers think the medication has a number of health advantages, including the following:

Treats Depression

It has been demonstrated to help those who are depressed by treating them. Additionally, it aids cognitive impairment. A study involving 24 participants revealed these intriguing possibilities.

Aids with Stroke

Because NSI 189 plays a part in neurogenesis or the production of new neurons, it can be utilized to treat stroke victims and restore damaged brain function and structure. Treatment with NSI 189 for 12 weeks significantly improved brain and motor function in rats with experimentally caused stroke. These benefits were determined to be long-lasting since they persisted 24 weeks after the medication was terminated. The effects of the medicine also completely overhauled the brain, improving overall performance for 6 months.

Assists in Cognitive Performance

In a research of 24 depressed individuals, it was found that it could improve cognitive performance. Although it is important to remember that this improvement is self-reported, 189 individuals’ cognitive functioning improved after receiving NSI treatment. Was this only the outcome of decreased depression brought on by the growth of additional neurons? It has been advised that more research be done on this.

Elevates Neuron Formation

One of the most exciting promises of NSI 189 is probably the creation of neurons. Curious minds have always been interested in the ability to repair fresh brain tissue. The hippocampus and amygdala regions of the brain can experience minor modifications, according to early animal research. Additionally, it can stop cell death brought on by an oxygen and glucose shortage.

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Start using NSI-189, a high-quality supplement that will help you achieve your goals


Side Effects

Animals have been used mostly in clinical research on NSI 189. Human subjects have been the subject of very little research. Patients may, however, experience negative effects at low or high doses. Patients who take the medication in doses of 40 mg might experience:

  • mouth ache
  • sleeplessness
  • headaches
  • dizziness
  • hyperthermia

Additional users claim to have encountered the following:

  • Fatigue
  • Drowsiness
  • Social anxiety
  • Angst and panic attacks
  • disregard for or carelessness with regard to important concerns like meeting deadlines
  • Headache
  • cognitive dysfunction
  • Anger
  • Sensation loss and nerve discomfort in the hands and feet 


There are two different ways to acquire NSE 189: in phosphate and in freebase forms. These vary mostly in taste and absorption rates. Less of the phosphate is effectively absorbed. They are put beneath the tongue and delivered sublingually. No matter which one you select, start at the extremely low amount to find the ideal dosage for you. Even at low doses, some users claim to experience the effects very immediately.

A startling rise in energy and a lift in mood have been noted by several users after taking just 7 mg of freebase sublingual. They also reported having more focus and mental clarity. Other users have primed the medication longer and more thoroughly, producing even more strong outcomes. These are usually followed by this.

  • Take NSI 189 sublingual 7 mg.
  • Prime as NSI is positioned beneath your tongue.
  • This has a significant favorable effect on feelings and general well-being.


Why is NSI-189 used by people?

People who self-experiment with NSI-189 frequently claim they do so to:

  • Mood
  • Memory
  • Learning
  • Mindfulness

How much NSI-189 should you dose, and how frequently?

NSI-189 probably won’t have significant effects until it’s taken every day for a while (4-12 weeks). Participants received doses ranging from 40 to 120 mg in research on the cognitive and emotional effects of NSI-189 in humans.

Should I eat before taking NSI-189?

The maximal blood concentration or overall concentration over time were unaffected by food. This indicates that the cognitive or affective effects of NSI-189 are probably unaffected by whether you take the medication on an empty stomach.

How can NSI-189 improve mood and thinking?

The effects of NSI-189 on the neuropsychopharmacological system have not yet been thoroughly elucidated by researchers. Here are a few of the mechanisms that scientists have looked at and discovered are involved in how NSI-189 affects mood and cognition:

  • increases the volume of the human right amygdala.
  • energizes neurogenesis
  • increases the levels of neurogenic substances including BDNF and SCF
  • increases synaptic plasticity (in mice)
  • increases mitochondrial activity and neurite development in rat primary sensory neurons.

Does NSI-189 offer neuroprotection?

It is likely that the neuroprotective benefits of NSI-189 depend on the component that is harming brain neurons. NSI-189 protected rats against radiation-induced cognitive impairment. NSI-189 may shield neurons from some types of neurochemical toxicity, but it won’t shield your brain from every possible threat.

How long does it take for the blood levels of NSI-189 to peak?

It takes NSI-189 1-2 hours to build up to its highest levels in human plasma.


A potential medication for the treatment of major depressive illness is NSI-189. Even though there was no human study, it was nevertheless considered to be a viable antidepressant drug following evaluation and even received FDA Orphan Drug Designation.

Additionally, it appears to have beneficial effects on the brain, which could be used to treat traumatic brain damage, age-related cognitive decline, or both. Medical professionals had great hopes following the phase I clinical trial’s substantial discovery of a potential new antidepressant.

To increase the effectiveness of NSI-189 as a new approach to treating depression and cognition disorder, other key factors must be covered and tested.

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Start using NSI-189, a high-quality supplement that will help you achieve your goals



The information provided above is not intended to substitute professional medical advice, diagnosis, or treatment. Always seek your physician’s advice or another qualified health provider with any questions you may have regarding a medical condition. Never disregard professional medical advice or delay in seeking it because of something you have seen or read. We bear no responsibility or liability for your use of any compound.